![]() ![]() The increase in day temperature (38.6☌) and afternoon relative humidity of 30.4% along with cloudy weather and intermittent rainfall favored the disease initiation and further spread of the disease. Secondary source of inoculum : Wind splashed rains.Primary source of inoculum:Infected cuttings.Spots on fruits were dark brown irregular slightly raised with oily appearance, which split open with L-shaped cracks under severe cases.Ĭausal organism: Xanthomonas axonopodies pv.punicae The pomegranate plantlets were screened for the bacterial blight spots on the leaves, and the severity of pomegranate blight disease on the leaves was determined by using a grade scale (Table 1), as mentioned by Anand et al.The pathogen also infects stem and branches causing girdling and cracking symptoms.At present this disease is observed on leaf, stem and on fruits, and has been responsible for the removing of many orchards in south India. Punicae, considered to be a minor disease, was reported as bacterial leaf spot in the 1950s. Owing to the non-availability of disease-resistant varieties of pomegranate, integrated disease management involving change of season, adequate nut. punicae, Xap is a serious threat to commercially successful pomegranate (Punica granatum L) crop. Appearance of one to several small water soaked, dark colored irregular spots on leaves resulting in premature defoliation under severe cases. Bacterial blight of pomegranate, caused by Xanthomonas axonopodis pv. Bacterial blight, caused by Xanthomonas axonopodis pv.Our data demonstrate that qPCR is more sensitive than other PCR methods along with being reliable for early diagnosis.Bacterial blight of pomegranate has assumed epidemic proportions (40-85% severity) in northern Karnataka (Bijapur, Bellary, Bagalkot) seriously threatening its cultivation. Initially, minute, circular water-soaked lesions are observed on undersurface of the foliage which later on are seen on both surfaces and become regular to irregular in shape and necrotic with brownish black centers and translucent yellow halo and vary in size between 1 mm. qPCR detected bacterial blight in orchards that did not show any disease symptoms. Bacterial blight can be diagnosed by symptoms which are conspicuous on leaves, twigs, and fruits. Field application of fungicides is necessary to control pathogens, which infect the fruit during developmental stages. However, conventional PCR-AGE detected pathogen at the onset of disease symptoms with a detection limit of 10 pg of bacterial DNA. A major bacterial disease of pomegranate is bacterial blight caused by Xanthomonas axanopodis pv. Pomegranate Bacterial Leaf Blight Bacterial blight of pomegranate has assumed epidemic proportions (40-85 severity) in northern Karnataka (Bijapur, Bellary, Bagalkot) seriously threatening its cultivation. PCR-CE and qPCR were capable of diagnosing bacterial blight 6 to 10 days before symptom appearance, with detection limits of 100 fg and 10 fg of bacterial DNA respectively. PCR coupled with agarose gel electrophoresis (PCR-AGE), PCR coupled with capillary electrophoresis (PCR-CE) and real-time PCR (qPCR) were applied for the early and accurate diagnosis of bacterial blight in pomegranate. DNA-based disease diagnostics using polymerase chain reaction (PCR) are reliable, precise, accurate and quick. This prediction system will do the detection immediately so that early detection will helps the farmer to. Bacterial blight in pomegranate leads to deceased yield (nearly by 90 ) and quality of fruits affecting the marketing value of fruits. Symptoms based disease diagnostic methods are labor-intensive, time-consuming and may not detect disease on asymptomatic plants. Pomegranate bacterial blight has become a very serious threat to pomegranate growers in the country. Precise and early diagnosis of bacterial blight is crucial for active surveillance and effective management of the disease. Bacterial blight drastically reduces the yield and quality of fruits, which are critical for pomegranate production. punicae is a major disease of pomegranate. Bacterial blight caused by Xanthomonas axonopodis pv. ![]()
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